Description
The E11 cell line has been meticulously cloned from the outgrowth of glomeruli, which were isolated from H-2kb-tsA58 transgenic mice. These transgenic mice carry a temperature-sensitive variant of the SV40 large T antigen, under the precise control of the IFN-g-inducible H-2kb promoter. This intricate genetic arrangement ensures the controlled development and sustained viability of the E11 cell line. One of the most remarkable attributes of the E11 cell line is its exceptional stability. Researchers have successfully propagated these cells for more than 40 passages without any observed phenotypic alterations. This enduring stability has facilitated rigorous experimentation and in-depth research, making the E11 cell line an ideal candidate for studies in podocyte biology. These cells exhibit stable expression of nephrin, a pivotal protein closely associated with podocytes. Furthermore, they display a remarkable capacity for forming abundant cell-cell contacts, a critical characteristic for mimicking the behavior of podocytes in their native environment. E11 cells not only stably express nephrin but also demonstrate the presence of a spectrum of other essential podocyte proteins. This comprehensive protein expression profile includes NEHP1, FAT, P-cadherin, podocin, CD2AP, ZO-1 (?-isoform), Lmx1b, podoplanin, synaptopodin, cortactin, and vimentin